| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.056781 |
| Chromosome: | chromosome 15 |
| Location: | 183090 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre15.g634650 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCTTCACAGCTTGCTAGACAGGTGATCTCTAGCATACGATACGCCTTCC |
| Internal bar code: | AGCAGTTTATGAGTGGGACTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3598 |
| LEAP-Seq percent confirming: | 95.7747 |
| LEAP-Seq n confirming: | 68 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 71 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGCTGGGAATTGGACGAGG |
| Suggested primer 2: | CTCAGCCCATACCGCTACAG |