| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.056784 |
| Chromosome: | chromosome 16 |
| Location: | 7823614 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g688526 | (1 of 1) PF00069//PF01846 - Protein kinase domain (Pkinase) // FF domain (FF) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGTCAGGAACACATTTACAGTACTTACTCTGCAACACGGGGCGAAGATG |
| Internal bar code: | TGGTATACCGGTTTTCACGTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2044 |
| LEAP-Seq percent confirming: | 35.0515 |
| LEAP-Seq n confirming: | 34 |
| LEAP-Seq n nonconfirming: | 63 |
| LEAP-Seq n unique pos: | 97 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGTGTTGCTCGTTTGCGTC |
| Suggested primer 2: | CGTCAATTTCGAGCGAGCAG |