| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.056785 |
| Chromosome: | chromosome 7 |
| Location: | 417916 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g314950 | RPC34 | DNA-directed RNA polymerase III subunit; (1 of 1) K03025 - DNA-directed RNA polymerase III subunit RPC6 (RPC6, POLR3F) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTGTGGAGCCGTGTCGCGGGTGGGGGCTGGGGGTTTGAAGATAGGGCGC |
| Internal bar code: | GAATGCCATAATGGTCGTCACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 835 |
| LEAP-Seq percent confirming: | 80.6452 |
| LEAP-Seq n confirming: | 25 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGAAGTGAAGTGCCGACGT |
| Suggested primer 2: | CCTCCTCCCAACCCCTGATA |