| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.056789 |
| Chromosome: | chromosome 10 |
| Location: | 4092282 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g448250 | (1 of 33) PF13855 - Leucine rich repeat (LRR_8) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTGCGCAGGATCCGCGCTAGAGGTCGTTGGGGCTGGTGTAGGGACGACG |
| Internal bar code: | ACTGGAGACATAATGTATTATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2930 |
| LEAP-Seq percent confirming: | 67.8571 |
| LEAP-Seq n confirming: | 19 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACACATTGTTCGCGTGTGG |
| Suggested primer 2: | TCTCATCGGTCGTATGCGTG |