Insertion junction: CLIP2.056792_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.056792
Chromosome:chromosome 10
Location:6675155
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g466400 (1 of 8) IPR011257 - DNA glycosylase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACCTGGGAACTCGGTGCATTTCGGGAATGGGGACTATTGTCGCGTGCCAC
Internal bar code:TCCGGGTGCGGGACTCGTCCTA

Confirmation - LEAP-Seq

LEAP-Seq distance:1410
LEAP-Seq percent confirming:88.8889
LEAP-Seq n confirming:8
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:AAGCCCCTGACATCCACATG
Suggested primer 2:GAGGGTTTGCAGCAAAGTCG