| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.056811 |
| Chromosome: | chromosome 17 |
| Location: | 5373189 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g738350 | PGA5,LPAAT2 | ER 1-acyl-sn-glycerol-3-phosphate acyltransferase; (1 of 2) PTHR10434 - 1-ACYL-SN-GLYCEROL-3-PHOSPHATE ACYLTRANSFERASE | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCTCTAGGTAGTAAACATGTCCGTGCTCACAAAGTTGCTGGGCCTGCC |
| Internal bar code: | TGACTCGGGGTCTTAGATTAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1313 |
| LEAP-Seq percent confirming: | 93.3333 |
| LEAP-Seq n confirming: | 14 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGAGAGGTCAACAGGTTGCT |
| Suggested primer 2: | CAATGTGACAACACCGCGTT |