| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.056819 |
| Chromosome: | plastome |
| Location: | 101843 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| CreCp.g802308 | ChreCp044,2716995,psbL | photosystem II reaction center subunit XII; (1 of 1) K02713 - photosystem II PsbL protein (psbL) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACAGTAAGGATAGCTTAAAATCCCTAAAGGGATCGTTATTTTACCTAGA |
| Internal bar code: | GCCAGGCACTATCGGTAGCTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 427 |
| LEAP-Seq percent confirming: | 55.2632 |
| LEAP-Seq n confirming: | 21 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 38 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTACTTAGGACGGCAGTGGC |
| Suggested primer 2: | GCGGCTGTGGCTGTAGATAT |