| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.056821 |
| Chromosome: | chromosome 8 |
| Location: | 2082083 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g369250 | FAP400 | Flagellar Associated Protein 400 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGGAATTGATCGCGGTACTCAAAGAATCGTGGCCTAACCCAGAATGCAG |
| Internal bar code: | AGGGGTGAATGGCAGGAAGGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1624 |
| LEAP-Seq percent confirming: | 82.3529 |
| LEAP-Seq n confirming: | 56 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 68 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGCAACATGAGTGGAGACC |
| Suggested primer 2: | GCAAGTAACCCACCTCCTCC |