| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.056827 |
| Chromosome: | chromosome 16 |
| Location: | 2019443 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g656851 | (1 of 2) PF07856 - Mediator of CRAC channel activity (Orai-1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGTGGCAACCCTGCATCTCATAATGCCTCGGCACAGTAACTCCGGCGTT |
| Internal bar code: | CTCCGTTAGACCCCGGACGTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5359 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 89 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 89 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGCTCTCGTGGGAGTTGTG |
| Suggested primer 2: | GTGGTACGCTGAGGATACGG |