Insertion junction: CLIP2.056855_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.056855
Chromosome:chromosome 2
Location:7165667
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g390430 (1 of 1) K13108 - smad nuclear-interacting protein 1 (SNIP1) 3'UTR
Cre09.g390467 CGL66 (1 of 3) PTHR12385:SF44 - PLASMA-MEMBRANE CHOLINE TRANSPORTER FAMILY PROTEIN; Conserved in the Green Lineage 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACATTGAAGTACTTGAATCAAGGAAGGAACTAATGTAGCGGCAGGGCTAC
Internal bar code:CAACCTGAGGGTGTTGGCTTAT

Confirmation - LEAP-Seq

LEAP-Seq distance:1724
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:80
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:80

Suggested primers for confirmation by PCR

Suggested primer 1:AGCTGTTGGAGAAGGACGTG
Suggested primer 2:AAGAGTGTGGGTTGGTGCAA