| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.056855 |
| Chromosome: | chromosome 2 |
| Location: | 7165667 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g390430 | (1 of 1) K13108 - smad nuclear-interacting protein 1 (SNIP1) | 3'UTR | |
| Cre09.g390467 | CGL66 | (1 of 3) PTHR12385:SF44 - PLASMA-MEMBRANE CHOLINE TRANSPORTER FAMILY PROTEIN; Conserved in the Green Lineage | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAAACAGTGGCGGGGGCGGTCTGTATGAATTGGACTTGGCCCCATCTCTA |
| Internal bar code: | TTACCCCCTCTCTCTCACCCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 742 |
| LEAP-Seq percent confirming: | 94.1176 |
| LEAP-Seq n confirming: | 48 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 51 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGAGTGTGGGTTGGTGCAA |
| Suggested primer 2: | AGCTGTTGGAGAAGGACGTG |