| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.056861 |
| Chromosome: | chromosome 16 |
| Location: | 2272804 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g658700 | FAP376 | (1 of 3) PF01661 - Macro domain (Macro); Flagellar Associated Protein 376 with ADP-ribose binding site | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTCTGGCACTACTAAATTTTCAGCACGCAGGGCGTGCGCTCGCTAGAGT |
| Internal bar code: | GAATCCTCTTGTATCAAAGCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2020 |
| LEAP-Seq percent confirming: | 90.0 |
| LEAP-Seq n confirming: | 27 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAGGTTGTGGCGTTGTGAG |
| Suggested primer 2: | ACACCCGGTTCATGTAGCTG |