Insertion junction: CLIP2.056864_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.056864
Chromosome:chromosome 16
Location:3003505
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g663876 (1 of 7) PF00415//PF13540 - Regulator of chromosome condensation (RCC1) repeat (RCC1) // Regulator of chromosome condensation (RCC1) repeat (RCC1_2) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGCAGGTTGAGACCTGCCGGTGTTGTCTGGTTAGCGACCTGTGCTGACGA
Internal bar code:GTCGACAGGATCCCCGAAATGC

Confirmation - LEAP-Seq

LEAP-Seq distance:4106
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:92
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:92

Suggested primers for confirmation by PCR

Suggested primer 1:AGGTGTTCAAAGTGGCCCAA
Suggested primer 2:CAGGCTTGTTGACTTGGTGC