Insertion junction: CLIP2.056865_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.056865
Chromosome:chromosome 10
Location:58776
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g417850 (1 of 1) IPR003613//IPR011047 - U box domain // Quinonprotein alcohol dehydrogenase-like superfamily 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AAACCGGGGCGATCACAACGCGGCGAGGAGCGCGAACCTGGCCGCTGAAG
Internal bar code:TGAGTAAAGCGACGAGTGTGCC

Confirmation - LEAP-Seq

LEAP-Seq distance:3406
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:10
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:CAGATGCTGCTGTTGATGCC
Suggested primer 2:TGCCTGTCGGTATTGTTGCT