| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.056867 |
| Chromosome: | chromosome 6 |
| Location: | 3594818 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278131 | (1 of 1) IPR000104//IPR002083//IPR008974 - Antifreeze protein, type I // MATH/TRAF domain // TRAF-like | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCCTTCTCCACACCTCCCACCCCGCCCAACACGCTGCCCTGCCAAGCCC |
| Internal bar code: | AGTTAATTATTCAGAACACGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1063 |
| LEAP-Seq percent confirming: | 80.0 |
| LEAP-Seq n confirming: | 36 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 45 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGGATGTCGAGCGGTCTAG |
| Suggested primer 2: | GCCGTTGGGGTACAGAATGA |