| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.056871 |
| Chromosome: | chromosome 2 |
| Location: | 7479357 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g392505 | (1 of 4) PTHR22849//PTHR22849:SF39 - WDSAM1 PROTEIN // SUBFAMILY NOT NAMED | 3'UTR | |
| Cre09.g392542 | (1 of 1) K11423 - histone-lysine N-methyltransferase SETD2 (SETD2, SET2) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGTTACATGCAAGCCCGCGCCACACGCCACACCCACAATACTTCCCACA |
| Internal bar code: | TGAAGAGGGCCCATCTGATGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1502 |
| LEAP-Seq percent confirming: | 43.3333 |
| LEAP-Seq n confirming: | 13 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTGTGTTTTACGTGTGCGC |
| Suggested primer 2: | GGCCTTTGCTTGCTTCACTC |