| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.056879 |
| Chromosome: | chromosome 6 |
| Location: | 3655026 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278145 | gene_edge/mRNA_edge/5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACACTAGTGTGGGCCTGTGCGTCGGTTCGCGGCTGGACGGACCGCACGG |
| Internal bar code: | ACTAAAACCGATACATTATTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1433 |
| LEAP-Seq percent confirming: | 85.7143 |
| LEAP-Seq n confirming: | 54 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 63 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCACCAGGTTGCAAAGACAG |
| Suggested primer 2: | GAAAAAGTGAGCGCAGGCAA |