| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.056908 |
| Chromosome: | chromosome 2 |
| Location: | 1734020 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g086200 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGATTGTTTGGGGCTTAATGCACGAGTAAAGGCCCCTGCCTTGTTGACGC |
| Internal bar code: | TGCTGTTTCGGCGTTCGAACTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4222 |
| LEAP-Seq percent confirming: | 56.1644 |
| LEAP-Seq n confirming: | 41 |
| LEAP-Seq n nonconfirming: | 32 |
| LEAP-Seq n unique pos: | 73 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTTCTCGCCCCTTGAAGTG |
| Suggested primer 2: | CTTACAGCCGGGAAGAACGT |