| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.056940 |
| Chromosome: | chromosome 7 |
| Location: | 887883 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g318651 | AOF4 | (1 of 1) 1.5.3.16 - Spermine oxidase / SMO; Flavin-containing amine oxidase | 5'UTR |
| Cre07.g318702 | (1 of 1) K11806 - WD repeat and SOF domain-containing protein 1 (WDSOF1) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTTCTTGTTCCGTTAGCCAGGTCAATGCTTATAGTTTCGAACAGCGGAA |
| Internal bar code: | ACGAGAAATAGGGTAATTCGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1557 |
| LEAP-Seq percent confirming: | 60.9756 |
| LEAP-Seq n confirming: | 25 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 41 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCCTCTAGGAAATCGTCGG |
| Suggested primer 2: | GTTGAAGCGGACCGAGGTAA |