| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.056940 |
| Chromosome: | chromosome 7 |
| Location: | 887895 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g318651 | AOF4 | (1 of 1) 1.5.3.16 - Spermine oxidase / SMO; Flavin-containing amine oxidase | 5'UTR |
| Cre07.g318702 | (1 of 1) K11806 - WD repeat and SOF domain-containing protein 1 (WDSOF1) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGGAAAACCCGATTTTCACGAGGACTCTTCTGCAGCGTTACCTTAGCTA |
| Internal bar code: | ACGAGAAATAGGGTAATTCGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1567 |
| LEAP-Seq percent confirming: | 85.7143 |
| LEAP-Seq n confirming: | 24 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTGAAGCGGACCGAGGTAA |
| Suggested primer 2: | GGCCTCTAGGAAATCGTCGG |