| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.056943 |
| Chromosome: | chromosome 12 |
| Location: | 7849195 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g553750 | SPL26 | Pre-mRNA splicing factor; (1 of 2) K13095 - splicing factor 1 (SF1) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGCACGTAGGCTGCTGCGCTGATCGAGAAGCTGCTTCAGCCCAACGACG |
| Internal bar code: | AGTACTTTAGCGGAAGTGGCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 193 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 4 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTACATACCCCGGCGTGTAC |
| Suggested primer 2: | TGGACTCGTTGACGGACTTG |