| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.056946 |
| Chromosome: | chromosome 1 |
| Location: | 8086818 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g063997 | (1 of 1) PF06741//PF14438 - LsmAD domain (LsmAD) // Ataxin 2 SM domain (SM-ATX) | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGAGCTCAGGCAGGACGAAATGGCGAGTTCGGAACGGACTGACCATGAC |
| Internal bar code: | CGTCTCGAATTCAAAGGGTTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 492 |
| LEAP-Seq percent confirming: | 51.8519 |
| LEAP-Seq n confirming: | 14 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTATCGCACGGTCATTGCAC |
| Suggested primer 2: | TTCATGGTGCTGGTACGCAT |