| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.056958 |
| Chromosome: | plastome |
| Location: | 82942 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| CreCp.g802298 | ChreCp033,rpoA,2717004 | (1 of 1) PF03118 - Bacterial RNA polymerase, alpha chain C terminal domain (RNA_pol_A_CTD); RNA polymerase alpha subunit | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGAGCGTTTAATGAACTTTGTAAATATAAATCCATAGTTCCACCGGGTA |
| Internal bar code: | GTTCGGATCGAGTACAAGGCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 135 |
| LEAP-Seq percent confirming: | 4.0 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 24 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGGTTGTTAGCATTCCACCA |
| Suggested primer 2: | CCCCTTCGGGCAAGTAAACT |