Insertion junction: CLIP2.056973_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.056973
Chromosome:chromosome 6
Location:2794729
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre06.g271650 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAGCTGCAGGAGGCCTACAGCGAGCACCAGAAGCGGATCAAGGGGGAGGC
Internal bar code:ACTGATACCTCTTTGACACTTG

Confirmation - LEAP-Seq

LEAP-Seq distance:113
LEAP-Seq percent confirming:33.3333
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:GTATGCCACGACCGCATCTA
Suggested primer 2:AGTGCTGGATGGTGTGTGAG