Insertion junction: CLIP2.056976_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.056976
Chromosome:plastome
Location:51377
Confidence (%):63
Locus systematic id Locus common name Defline Feature
CreCp.g802285 psbA,ChreCp021,2716969 (1 of 2) K02703 - photosystem II P680 reaction center D1 protein (psbA); photosystem II protein D1 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TAGTCTAAACTTAATAGAAATATTAAGATCCACTTTTGCGTGAGTGGGAA
Internal bar code:GTACGAGAATGTTGGCTATGAT

Confirmation - LEAP-Seq

LEAP-Seq distance:133
LEAP-Seq percent confirming:7.14286
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:13
LEAP-Seq n unique pos:14

Suggested primers for confirmation by PCR

Suggested primer 1:AACTGCCTGCTTCCTCCTTC
Suggested primer 2:CCGGATCGTGCAGCTAGAAA