| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.057001 |
| Chromosome: | chromosome 11 |
| Location: | 607315 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467609 | (1 of 3) K10587 - ubiquitin-protein ligase E3 A (UBE3A, E6AP) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCAATTTGAAGGAGGACTTCCGGAAATGGAAACACGTGAGTCAGGAGGCA |
| Internal bar code: | GTGGATTTCGGCGCTTTATCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1013 |
| LEAP-Seq percent confirming: | 90.625 |
| LEAP-Seq n confirming: | 29 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 32 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGTGTGGTTTTGTGGCGTC |
| Suggested primer 2: | AAACTCACCTGTCTCAGCCG |