| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.057002 |
| Chromosome: | chromosome 12 |
| Location: | 3394971 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g497900 | (1 of 5) IPR000104//IPR016024 - Antifreeze protein, type I // Armadillo-type fold | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGAGTAGTGCATGAGGTCAACAAGCTTGTGAGGCTGAGTTGCAAGTGACC |
| Internal bar code: | CAGGTGTCCCGCTGTGTTCGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1272 |
| LEAP-Seq percent confirming: | 97.2222 |
| LEAP-Seq n confirming: | 35 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 36 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTCGTACAGGAGGGGGAGA |
| Suggested primer 2: | TCATTCACAGCGTCCAGTCC |