| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.057002 |
| Chromosome: | chromosome 12 |
| Location: | 3394974 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g497900 | (1 of 5) IPR000104//IPR016024 - Antifreeze protein, type I // Armadillo-type fold | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTCTTGCCACCACCCTCACTAACGCCAGCATCACCGTTCCGAGCCCAAA |
| Internal bar code: | CAGGTGTCCCGCTGTGTTCGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1399 |
| LEAP-Seq percent confirming: | 33.3333 |
| LEAP-Seq n confirming: | 5 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCATTCACAGCGTCCAGTCC |
| Suggested primer 2: | CTTCGTACAGGAGGGGGAGA |