| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.057008 |
| Chromosome: | chromosome 12 |
| Location: | 6465003 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g537581 | (1 of 1) 3.5.4.10 - IMP cyclohydrolase / Inosinicase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATCAGAACCCCGTCCTAGTTTATGAAGCAGAGACAATCGTCAAACACAC |
| Internal bar code: | TACGTCATGAGGATTTTACGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 835 |
| LEAP-Seq percent confirming: | 86.3636 |
| LEAP-Seq n confirming: | 38 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 44 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTGTGCAATAAGTACGGCG |
| Suggested primer 2: | CTGGTTGGCCACACGAAATG |