| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.057020 |
| Chromosome: | chromosome 9 |
| Location: | 3541401 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g396475 | (1 of 1) PTHR24202//PTHR24202:SF4//PTHR24202:SF52 - FAMILY NOT NAMED // E3 UBIQUITIN-PROTEIN LIGASE MIB2 // E3 UBIQUITIN-PROTEIN LIGASE MIB1 | 5'UTR_intron | |
| Cre09.g396485 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCTCAACGAGTCTCACCGTCTCGGTCTCCGCGGTCCTCTTAATTTCCTC |
| Internal bar code: | CCTATGTTCCTGATGTATAATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 438 |
| LEAP-Seq percent confirming: | 85.7143 |
| LEAP-Seq n confirming: | 6 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTACGAAGAGGACGTAGCCG |
| Suggested primer 2: | TTGTCAAGTAGCGCCAGGAG |