| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.057042 |
| Chromosome: | chromosome 17 |
| Location: | 1558682 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g707137 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCGCATATGTGTACGTACGTCAGCCATGCGTACAGCTTCTTATCAACAC |
| Internal bar code: | GTGCGACTATCTAGTGCTATCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 595 |
| LEAP-Seq percent confirming: | 12.5 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATTTGGTCGCGTTTGGGTG |
| Suggested primer 2: | TCAGTCGTCTAGGTCCAGCA |