Insertion junction: CLIP2.057043_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.057043
Chromosome:chromosome 6
Location:7759871
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g303000 (1 of 24) IPR006553 - Leucine-rich repeat, cysteine-containing subtype 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCTGCCGTGACATGCACTGCAACGGCACACTCGCAAACGCCAAGCGGTC
Internal bar code:TATATACGGCGTATACGATCTA

Confirmation - LEAP-Seq

LEAP-Seq distance:362
LEAP-Seq percent confirming:91.6667
LEAP-Seq n confirming:11
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:CTGCCTGGTCGTCATTTCCT
Suggested primer 2:CATCATTGTCGACGTCGCAC