| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.057044 |
| Chromosome: | chromosome 10 |
| Location: | 853725 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g423550 | ATA2 | threonine aldolase; (1 of 1) 4.1.2.49 - L-allo-threonine aldolase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCCAGGACGGTGGGCTAGGGTTTGGCATGGCATACAGCAGGCACGAGCA |
| Internal bar code: | GTTGATGATAGTGAATCAACGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 953 |
| LEAP-Seq percent confirming: | 7.69231 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 24 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGACAAAAACAGAAGCGGCA |
| Suggested primer 2: | CCAGGTTTGCGTGAGTTTGG |