| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.057076 |
| Chromosome: | chromosome 10 |
| Location: | 2212392 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| intergenic |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTGGATGTGGGCTTCAGGAGGCGCTGGCGGCTTGCCCATTCCCTTCAGC |
| Internal bar code: | GCAGTTAAAGGGCGCGGATCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 247 |
| LEAP-Seq percent confirming: | 82.1429 |
| LEAP-Seq n confirming: | 23 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAGTCCCTTGAAGCTCCAG |
| Suggested primer 2: | CGATGGATCTGCGCTTTGTG |