Insertion junction: CLIP2.057083_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.057083
Chromosome:chromosome 16
Location:139394
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g695250 (1 of 726) IPR011009 - Protein kinase-like domain 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTTGTTGTTTAGGGTTACAACCCCATTATTAAATACAAGAGTGGTGCTCT
Internal bar code:AAAATGATTGGCACAGTTGTAT

Confirmation - LEAP-Seq

LEAP-Seq distance:735
LEAP-Seq percent confirming:94.7368
LEAP-Seq n confirming:18
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:19

Suggested primers for confirmation by PCR

Suggested primer 1:CGACACACTGAGGCTCAAGT
Suggested primer 2:TGTCGCCACATCGATACGAG