| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.057085 |
| Chromosome: | plastome |
| Location: | 127467 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| CreCp.g802316 | ChreCp052,2717043,ycf10,cemA | (1 of 1) PTHR33650:SF2 - CHLOROPLAST ENVELOPE MEMBRANE PROTEIN; heme-binding protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGATGGCAACATTTCACCCTCTGCGGAAGGTAAATAAATTTGTCGCAAGT |
| Internal bar code: | TAGTGCTTGATTGAGCTTTGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 416 |
| LEAP-Seq percent confirming: | 33.3333 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCCATCACCAAACTCATCGT |
| Suggested primer 2: | GCAGCAGTACCTTGACCCAT |