Insertion junction: CLIP2.057094_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.057094
Chromosome:chromosome 12
Location:5511347
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g530250 3'UTR
Cre12.g530300 FKB16C,FKB6,FKB16-3 (1 of 1) PTHR10516//PTHR10516:SF264 - PEPTIDYL-PROLYL CIS-TRANS ISOMERASE // FK506-BINDING PROTEIN 1; peptidyl-prolyl cis-trans isomerase, FKBP-type 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCAACAACCCGTTCTTGCCGATTCTACCTTTCAACAACGCAAGATTGCGT
Internal bar code:ACTCAATTACCCTAGGTGTACC

Confirmation - LEAP-Seq

LEAP-Seq distance:5028
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:32
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:32

Suggested primers for confirmation by PCR

Suggested primer 1:CCTTCCACCTGTACGACACC
Suggested primer 2:GAGGCGGGAGGTTGAGAAAA