Insertion junction: CLIP2.057101_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.057101
Chromosome:chromosome 10
Location:4763869
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g452750 TPT18,TPT16 UMAMIT (Usually Multiple Acids Move In and Out Transporters) type transporter; (1 of 3) PTHR11132//PTHR11132:SF125 - SOLUTE CARRIER FAMILY 35 // SUBFAMILY NOT NAMED 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAGGGGGAGATGGGCTGAGCTCTGCCCCAGTCAGTTAAGTGGTGACACTG
Internal bar code:AGCTTTCTTCGATTGTAGTTTA

Confirmation - LEAP-Seq

LEAP-Seq distance:1959
LEAP-Seq percent confirming:80.0
LEAP-Seq n confirming:36
LEAP-Seq n nonconfirming:9
LEAP-Seq n unique pos:45

Suggested primers for confirmation by PCR

Suggested primer 1:TTGACGAGTGTGACGGACAG
Suggested primer 2:GTGGGGCCGCATCTATCTAC