| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.057102 |
| Chromosome: | chromosome 13 |
| Location: | 4246757 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g591951 | 3'UTR | ||
| Cre13.g592000 | (1 of 19) K08850 - aurora kinase, other [EC:2.7.11.1] (AURKX) | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTTGGAGTAGTGGCTGTGAAACAATGAGCGTGGCTGTTGCTGGACGTTG |
| Internal bar code: | ATACCTATCTTTCAATATATAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1683 |
| LEAP-Seq percent confirming: | 96.6667 |
| LEAP-Seq n confirming: | 29 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGAAGGTTACAAGGCGCGA |
| Suggested primer 2: | GGTGAACTGGATGCTCAGCT |