Insertion junction: CLIP2.057112_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.057112
Chromosome:chromosome 1
Location:3501344
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g022800 FAL1 Similar to Flagellar Associated Protein FAP159 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TAGCACCGACAGGTCCCCTCCCCCACCTGCTGCATGTTACGTGTGTTTGC
Internal bar code:GGTCTCAGCACCTTATACCAAT

Confirmation - LEAP-Seq

LEAP-Seq distance:1832
LEAP-Seq percent confirming:89.3617
LEAP-Seq n confirming:42
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:47

Suggested primers for confirmation by PCR

Suggested primer 1:GGTGTCAAATATTCGGCCGC
Suggested primer 2:ACGGACATAGGGCGCTAATG