| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.057127 |
| Chromosome: | chromosome 10 |
| Location: | 5039623 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g454800 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCACGGGGTAGCTACGCCTTGCCACACCTTGTGCTATTCAGCACACGGG |
| Internal bar code: | CCCTGGACTACGACTCGATTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 998 |
| LEAP-Seq percent confirming: | 96.2963 |
| LEAP-Seq n confirming: | 26 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGAGCAACTACTAGCGGAG |
| Suggested primer 2: | AGAACCAGAACCAGCCACAG |