| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.057142 |
| Chromosome: | chromosome 17 |
| Location: | 1070472 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g703700 | SCLB1,SCL2,SUCLA2 | Succinate-CoA ligase beta chain; (1 of 1) K01900 - succinyl-CoA synthetase beta subunit (LSC2) | 5'UTR |
| Cre17.g703750 | (1 of 1) PF14222 - Cell morphogenesis N-terminal (MOR2-PAG1_N) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTTAGGCGTGGTAATGGCAAGTTTCGGGGATGCATGCGACTTGAGCCAA |
| Internal bar code: | ATGCGGCTTCGAGAGTAATGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 642 |
| LEAP-Seq percent confirming: | 15.0685 |
| LEAP-Seq n confirming: | 11 |
| LEAP-Seq n nonconfirming: | 62 |
| LEAP-Seq n unique pos: | 73 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAGTCTGCAGCACCTGTAA |
| Suggested primer 2: | CAGCACTCTGACAGCCTGAA |