| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.057151 |
| Chromosome: | chromosome 7 |
| Location: | 1035975 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g319650 | FXL4,FXL5 | FixL-like PAS domain protein; (1 of 3) IPR000014//IPR013767 - PAS domain // PAS fold | 5'UTR |
| Cre07.g319701 | (1 of 1) PTHR10071//PTHR10071:SF184 - TRANSCRIPTION FACTOR GATA GATA BINDING FACTOR // GATA TRANSCRIPTION FACTOR 10-RELATED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTATCCGGCATGAGATACCGGTATGACGCGCTGCTAGCTCTTGACCTGGG |
| Internal bar code: | TGTTTCTGATGTGACTCCCAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1959 |
| LEAP-Seq percent confirming: | 92.5 |
| LEAP-Seq n confirming: | 74 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 80 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAGCCTTATAAAAGCCGCC |
| Suggested primer 2: | CGTCGGGCATGATACGAAGA |