| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.057153 |
| Chromosome: | chromosome 16 |
| Location: | 6085980 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g677550 | CYA15 | (1 of 8) PF00211//PF01547 - Adenylate and Guanylate cyclase catalytic domain (Guanylate_cyc) // Bacterial extracellular solute-binding protein (SBP_bac_1); Solute-binding protein-like adenylate cyclase | 5'UTR |
| Cre16.g677600 | SOM5 | S-adenosyl-L-methionine-dependent O-methyltransferase; (1 of 4) PTHR13600//PTHR13600:SF12 - LEUCINE CARBOXYL METHYLTRANSFERASE // LEUCINE CARBOXYL METHYLTRANSFERASE | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGACATGTCCTAGAATGTTGAGCCCCGCTCATCAACTAACTCGAGCCTA |
| Internal bar code: | CACTGTTAATGTCTCTGATGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3372 |
| LEAP-Seq percent confirming: | 46.3415 |
| LEAP-Seq n confirming: | 19 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 41 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGTAGTAGCTGTGGCCGTA |
| Suggested primer 2: | AACATTCACGTCGGTCCGAA |