Insertion junction: CLIP2.057160_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.057160
Chromosome:chromosome 7
Location:1731348
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre07.g325717 (1 of 7) 2.4.99.18 - Dolichyl-diphosphooligosaccharide--protein glycotransferase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCCATAGTGCCATGGTGTTGGTCGCTGGTGGGGCGGATGGAGGGTGGTG
Internal bar code:GTGTCACCGGGGCTCGTGTTTG

Confirmation - LEAP-Seq

LEAP-Seq distance:1876
LEAP-Seq percent confirming:47.3684
LEAP-Seq n confirming:9
LEAP-Seq n nonconfirming:10
LEAP-Seq n unique pos:19

Suggested primers for confirmation by PCR

Suggested primer 1:TTCCGCAACACTACTCCACC
Suggested primer 2:TGGAGCAACAAGTAGGCGAG