| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.057169 |
| Chromosome: | chromosome 12 |
| Location: | 6270973 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g536450 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCCGAGCCATCACATGCTATCAGGGCCCAACCCCGACTTTGCTGCAAAC |
| Internal bar code: | GTTGTGTCGGTAGTTCCACTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4758 |
| LEAP-Seq percent confirming: | 55.303 |
| LEAP-Seq n confirming: | 73 |
| LEAP-Seq n nonconfirming: | 59 |
| LEAP-Seq n unique pos: | 132 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGTTTTGGGACTGCACTGT |
| Suggested primer 2: | CGGTCTGCCCTTTGAGCTTA |