| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.057187 |
| Chromosome: | chromosome 12 |
| Location: | 5964248 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g533950 | COT1 | Cobalt transport protein; (1 of 1) PTHR33514//PTHR33514:SF3 - FAMILY NOT NAMED // PROTEIN ABCI12, CHLOROPLASTIC | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGATTTGTGCCGCAAAGGAGCGTCCATTGACGGCAGTGCAATTGTGCA |
| Internal bar code: | TGTGTCGGATCACCATAGTGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1829 |
| LEAP-Seq percent confirming: | 85.7143 |
| LEAP-Seq n confirming: | 18 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCAGGTTGAGTGGTTGTGT |
| Suggested primer 2: | TAGGCTATGACGCAGCATGG |