Insertion junction: CLIP2.057189_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.057189
Chromosome:chromosome 12
Location:5163778
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre12.g526850 HEL56 (1 of 1) K14810 - ATP-dependent RNA helicase DDX56/DBP9 [EC:3.6.4.13] (DDX56, DBP9); DEAD box ATP-dependent RNA helicase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACGCACGCACGCACGCACGCACGCACGCACACAACACGTGATGCCCCGCC
Internal bar code:CTGTAGATCACTCCGCACGGCA

Confirmation - LEAP-Seq

LEAP-Seq distance:370
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:ACAGAAGATGTGCGTGGGAG
Suggested primer 2:TCCCTCGGTTTACTTTGGGC