Insertion junction: CLIP2.057191_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.057191
Chromosome:chromosome 13
Location:3850527
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g589450 FBB6L1 (1 of 1) IPR002035//IPR013694 - von Willebrand factor, type A // VIT domain; FBB6 like protein 1 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AACCTGGAGGCGCACACCGAGGTGACGGTGGAGTTCAGCTACCTCCGGCT
Internal bar code:TTGTTTGTCCTCGAGTGGACGG

Confirmation - LEAP-Seq

LEAP-Seq distance:1055
LEAP-Seq percent confirming:44.4444
LEAP-Seq n confirming:8
LEAP-Seq n nonconfirming:10
LEAP-Seq n unique pos:18

Suggested primers for confirmation by PCR

Suggested primer 1:CAGGCAAGCAGCTAGGAACT
Suggested primer 2:CTACATGCAAACGAGCGCTC