Insertion junction: CLIP2.057198_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.057198
Chromosome:chromosome 12
Location:5134893
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g526264 (1 of 10) IPR000511 - Cytochrome c/c1 haem-lyase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGACGATGCTTCGACGGGGCATCCCGCCCCCGCCCATGTTCATGCGTCC
Internal bar code:TGTGACGAGGGCTTCAGTCACG

Confirmation - LEAP-Seq

LEAP-Seq distance:1200
LEAP-Seq percent confirming:40.0
LEAP-Seq n confirming:4
LEAP-Seq n nonconfirming:6
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:TGTGTGGGTTAGAGTGCGTC
Suggested primer 2:GCAGTGGGCGCAACAAATAA